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ccnd2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc ccnd2
    Ccnd2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-ccnd2/Cyclin+D2+Rabbit+mAb/pm41411970-75-66-67
    Average 95 stars, based on 246 article reviews
    ccnd2 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: Antibodies and chemicals The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Transfection:

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: Antibodies and chemicals The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Flow Cytometry:

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: Antibodies and chemicals The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Cell Cycle Assay:

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: Antibodies and chemicals The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Knockdown:

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: Antibodies and chemicals The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Western Blot:

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: Antibodies and chemicals The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Labeling:

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: Antibodies and chemicals The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Control:

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: Antibodies and chemicals The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Cell Culture:

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: Antibodies and chemicals The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Cell Counting:

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: Antibodies and chemicals The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Colony Assay:

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: Antibodies and chemicals The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).

    Article Title: Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1
    Article Snippet: The following antibodies (Abs) were used: rabbit anti-CCND1 [Cat. No. 2922 for IP/immunoblotting (IB) and 2978 for immunohistochemistry (IHC), CST, Danvers, MA, USA], rabbit anti-USP5 (Cat. No. 10473-1-AP, Proteintech, Wuhan, Hubei, China), rabbit anti-CCND2 (Cat. No. 2924, CST), rabbit anti-CCND3 (Cat. No. 26775-1-AP, Proteintech), mouse anti-CDK4 (Cat. No. 12790, CST), mouse anti-CDK6 (Cat. No. 3136, CST), mouse anti-Rb (Cat. No. 9309, CST), rabbit anti-Phospho-Rb (Ser780) (Cat. No. 3590, CST), rabbit anti-β-tubulin (Cat. No. 10068-1-AP, Proteintech), mouse anti-Ub (Cat. No. sc-271289, Santa Cruz, Dallas, TX, USA), mouse anti-Myc (Cat. No. M192-3, MBL, Tokyo, Japan), mouse anti-Flag (Cat. No. M185-3, MBL), mouse anti-HA (Cat. No. M180-3, MBL), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H+L) (Cat. No. A0208, Beyotime) and anti-mouse IgG (H+L) (Cat. No. A0216, Beyotime).



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    A - Electropherogram showing both alleles (top and bottom) of CCND2 biallelic frameshift line CCND2 FS-T1 compared to wildtype (middle) causing truncation at positions 266 and 272 on allele 1 and allele 2, respectively. B - illustrates the same but for the second CCND2 biallelic frameshift line CCND2 FS-T2 showing an 11 bp deletion on allele 1 (top) and a different 11 bp deletion on allele 2 (bottom) compared to wildtype (middle), causing CCND2 truncation at position 272 and 274 respectively. C - Sanger sequencing electropherograms indicating with arrows the single base pair change c.814G>T in heterozygous (top) and homozygous (bottom) CCND2 E272*Het and CCND2 E272*Hom CRISPR-Cas9 edited lines compared to wildtype (middle).

    Journal: bioRxiv

    Article Title: Nonsense, but not frameshift, truncating mutations result in Cyclin D2 stabilisation in an induced pluripotent stem cell model of MPPH

    doi: 10.1101/2025.08.29.673076

    Figure Lengend Snippet: A - Electropherogram showing both alleles (top and bottom) of CCND2 biallelic frameshift line CCND2 FS-T1 compared to wildtype (middle) causing truncation at positions 266 and 272 on allele 1 and allele 2, respectively. B - illustrates the same but for the second CCND2 biallelic frameshift line CCND2 FS-T2 showing an 11 bp deletion on allele 1 (top) and a different 11 bp deletion on allele 2 (bottom) compared to wildtype (middle), causing CCND2 truncation at position 272 and 274 respectively. C - Sanger sequencing electropherograms indicating with arrows the single base pair change c.814G>T in heterozygous (top) and homozygous (bottom) CCND2 E272*Het and CCND2 E272*Hom CRISPR-Cas9 edited lines compared to wildtype (middle).

    Article Snippet: : Rabbit-CCND2 (CST, 1/1,000) and Mouse-B-actin (Ambion, 1/20,000) antibodies were used.

    Techniques: Sequencing, CRISPR

    A - 2D genomic and proteomic structure of CCND2, showing location of MPPH disease causing variants at the C-terminus (bottom) ( Mirzaa et al ., 2014 , Zhao et al ., 2024 ) and likely pathogenic variants from ClinVar in italics (top). Green indicates phosphorylation sites Ser269, Ser271 and Thr280, pink indicates ubiquitination site Lys270. B – Illustration of the CCND2 C- terminus showing the site of truncations, at the gene and protein level, in each CRISPR-Cas9 engineered line in comparison to wildtype (WT). Red hatched regions indicate regions of amino acid sequence changes due to a frameshift mutation. Green and pink highlighted regions signify locations of phosphorylation sites (Ser269, Ser271 and Thr280) and ubiquitination site Lys270, respectively.

    Journal: bioRxiv

    Article Title: Nonsense, but not frameshift, truncating mutations result in Cyclin D2 stabilisation in an induced pluripotent stem cell model of MPPH

    doi: 10.1101/2025.08.29.673076

    Figure Lengend Snippet: A - 2D genomic and proteomic structure of CCND2, showing location of MPPH disease causing variants at the C-terminus (bottom) ( Mirzaa et al ., 2014 , Zhao et al ., 2024 ) and likely pathogenic variants from ClinVar in italics (top). Green indicates phosphorylation sites Ser269, Ser271 and Thr280, pink indicates ubiquitination site Lys270. B – Illustration of the CCND2 C- terminus showing the site of truncations, at the gene and protein level, in each CRISPR-Cas9 engineered line in comparison to wildtype (WT). Red hatched regions indicate regions of amino acid sequence changes due to a frameshift mutation. Green and pink highlighted regions signify locations of phosphorylation sites (Ser269, Ser271 and Thr280) and ubiquitination site Lys270, respectively.

    Article Snippet: : Rabbit-CCND2 (CST, 1/1,000) and Mouse-B-actin (Ambion, 1/20,000) antibodies were used.

    Techniques: Phospho-proteomics, Ubiquitin Proteomics, CRISPR, Comparison, Sequencing, Mutagenesis

    A - Representative fluorescence images of pluripotency markers on wildtype and mutant iPSC lines, stained for Oct 3/4 (green), SSEA4 (red) and Hoechst (blue). B - Representative western blot of CCND2 in the presence or absence of cycloheximide in CCND2 FS-T1 and CCND2 FS-T2 frameshift lines. C - Representative western blot of CCND2 in the presence or absence of cycloheximide in CCND2 E272*Het and CCND2 E272*Hom nonsense lines.

    Journal: bioRxiv

    Article Title: Nonsense, but not frameshift, truncating mutations result in Cyclin D2 stabilisation in an induced pluripotent stem cell model of MPPH

    doi: 10.1101/2025.08.29.673076

    Figure Lengend Snippet: A - Representative fluorescence images of pluripotency markers on wildtype and mutant iPSC lines, stained for Oct 3/4 (green), SSEA4 (red) and Hoechst (blue). B - Representative western blot of CCND2 in the presence or absence of cycloheximide in CCND2 FS-T1 and CCND2 FS-T2 frameshift lines. C - Representative western blot of CCND2 in the presence or absence of cycloheximide in CCND2 E272*Het and CCND2 E272*Hom nonsense lines.

    Article Snippet: : Rabbit-CCND2 (CST, 1/1,000) and Mouse-B-actin (Ambion, 1/20,000) antibodies were used.

    Techniques: Fluorescence, Mutagenesis, Staining, Western Blot

    Sections were stained for PAX6, β-Tubulin, TBR1, NeuN and Hoechst on all CCND2 CRISPR-Cas9 edited lines and compared to wildtype. For each, similar results were obtained from 2 independent batches grown. Scale bar 20um at x10 magnification.

    Journal: bioRxiv

    Article Title: Nonsense, but not frameshift, truncating mutations result in Cyclin D2 stabilisation in an induced pluripotent stem cell model of MPPH

    doi: 10.1101/2025.08.29.673076

    Figure Lengend Snippet: Sections were stained for PAX6, β-Tubulin, TBR1, NeuN and Hoechst on all CCND2 CRISPR-Cas9 edited lines and compared to wildtype. For each, similar results were obtained from 2 independent batches grown. Scale bar 20um at x10 magnification.

    Article Snippet: : Rabbit-CCND2 (CST, 1/1,000) and Mouse-B-actin (Ambion, 1/20,000) antibodies were used.

    Techniques: Staining, CRISPR

    Journal: iScience

    Article Title: circGlis3 promotes β-cell dysfunction by binding to heterogeneous nuclear ribonucleoprotein F and encoding Glis3-348aa protein

    doi: 10.1016/j.isci.2023.108680

    Figure Lengend Snippet:

    Article Snippet: Rabbit monoclonal anti-CCND2 , Cell Signaling Technology , Cat# 3741; RRID: AB_2070685.

    Techniques: Virus, Recombinant, Modification, Lysis, Transfection, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, CCK-8 Assay, Imaging, TUNEL Assay, Apoptosis Assay, In Situ Hybridization, Immunoprecipitation, Mass Spectrometry, Western Blot, shRNA, Plasmid Preparation, Software